Pisum sativum unigene v1

Analysis NamePisum sativum unigene v1
Unigene NamePisum sativum unigene v1
Methodcap3 (1.0)
SourcePisum sativum unigene v1
Date performed2010-12-27
Number of Reads17,717
Number of Contigs3,095
Number of Singlets6,950
OrganismsPisum sativum

An assembly of 18,552 EST sequences downloaded from NCBI dbEST using the cap3 assembly software (parameter: -p 90). 17,717 ESTs were used in the analysis after trimming using crossmatch against UniVec_Core (parameter: -minmatch 10 -penalty -2 -minscore 20 -screen) and filtering out rRNA/tRNA contamination using tblastx (parameter: -e 1e-6). Poly-A tail was also trimmed after RNA filtering. The assembly results in 3,095 contigs and 6,950 singlets.